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ytvA [2018-02-13T06:35:42.000Z]
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ytvA [2018-02-13T06:35:42.000Z]

blue light sensor, positive regulation of SigB activity under conditions of blue light
locus
BSU30340
pI
4.69
mw
29.04 kDa
protein length
261 aa Sequence Blast
gene length
783 bp Sequence Blast
function
control of SigB activity
product
blue light receptor, flavoprotein
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]

    • Gene

    Coordinates
    3,106,210 → 3,106,995

    The protein

    Catalyzed reaction/ biological activity

  • mediates light-dependent stimulation of [protein|ADC52A22950736A0435AEEEC43F7407878786A81|RsbT] kinase activity [Pubmed|23416074]

    • Paralogous protein(s)

  • [protein|621357D1FB6AC1B0499732EFE226F982B26CE34E|RsbR], [protein|F53C527995909A1CFA72C7BF919DD10EE175C6D7|RsbRB], [protein|5EEEDE60CE9E5CDAEBAB0E03AABDF1DF62F1F002|RsbRC], [protein|979D7A45EAD97C99015029400A85795061BAA367|RsbRD], [protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|YtvA]

    • [SW|Domains]

  • [SW|PAS domain]
  • LOV domain, STAS domain

    • [SW|Cofactors]

  • FMN

    • Effectors of protein activity

  • [protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|YtvA]-dependent light activation of the [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB] stress response is positively and negatively regulated by [protein|621357D1FB6AC1B0499732EFE226F982B26CE34E|RsbR] and [protein|F53C527995909A1CFA72C7BF919DD10EE175C6D7|RsbRB], respectively [Pubmed|22287516]

    • Structure

  • [PDB|2PR5] (dark), [PDB|2PR6] (light), [PDB|2MWG]

    • Expression and Regulation

    Operons

    genes
    [gene|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|16923906], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A136 (ytvA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/136 NBRP B. subtilis, Japan]
  • 1A965 ( ''ytvA''::''erm''), [Pubmed|11157946], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A965&Search=1A965 BGSC]
  • BKE30340 (Δ[gene|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAATCCCCCTTAGGC, downstream forward: _UP4_TAAAAAGATCCCGCTCACCC
  • BKK30340 (Δ[gene|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAATCCCCCTTAGGC, downstream forward: _UP4_TAAAAAGATCCCGCTCACCC

    • Expression vector

  • pGP2685, expression of His-[protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|YtvA] in ''E. coli'' (based on [SW|pGP570]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Chet Price], Davis, USA [http://foodscience.ucdavis.edu/price_lab/pricelab.html homepage]

    • References

    Reviews

  • 18400553,21822294,26189730,28271471

    • Original publications

  • 27203230,19581299,16923906,17575448,17764689,17443319,17200735,16923909,16797012,16022561,15339223,11964249,11157946,19891470,20062844,19783626,20800068,22247770,23047813,23416074,23456923,23817467,21259411,21388385,21410163,21851109,22287516,23861663,23828427,23900620,23940057,24171435,24278408,25211155,26402155